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ProductsBack/Eph receptor B1 (EPHB1) Mouse Monoclonal Antibody [Clone ID: 5F10A4]
Western blot analysis using EhpB1 antibody Cat.-No AM06214SU-N against MDA-MB-468 (Lane 1), MDA-MB-453 (Lane 2), MCF-7 (Lane 3), T47D (Lane 4) and SKBR-3 (Lane 5) cell lysate.
Western blot analysis using EhpB1 antibody Cat.-No AM06214SU-N against MDA-MB-468 (Lane 1), MDA-MB-453 (Lane 2), MCF-7 (Lane 3), T47D (Lane 4) and SKBR-3 (Lane 5) cell lysate.
Western blot analysis using EhpB1 antibody Cat.-No AM06214SU-N against MDA-MB-468 (Lane 1), MDA-MB-453 (Lane 2), MCF-7 (Lane 3), T47D (Lane 4) and SKBR-3 (Lane 5) cell lysate.

Eph receptor B1 (EPHB1) Mouse Monoclonal Antibody [Clone ID: 5F10A4]

AM06214SU-N
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetEPHB1
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Eph receptor B1 (EPHB1) Mouse Monoclonal Antibody [Clone ID: 5F10A4]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5F10A4
  • Gene ID2047
  • Target name
    EPHB1
  • Target description
    EPH receptor B1
  • Target synonyms
    ELK, EPHT2, Hek6, NET, ephrin type-B receptor 1, EK6, EPH-like kinase 6, eph tyrosine kinase 2, neuronally-expressed EPH-related tyrosine kinase, tyrosine-protein kinase receptor EPH-2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP54762
  • Protein Name
    Ephrin type-B receptor 1
  • Scientific Description
    Eph receptor B1 (EPHB1) mouse monoclonal antibody, clone 5F10A4, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Eph receptor B1 using anti-Eph receptor B1 antibody (PB9584). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human Hel whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph receptor B1 antigen affinity purified polyclonal antibody (Catalog # PB9584) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph receptor B1 at approximately 120 kDa. The expected band size for Eph receptor B1 is at 110 kDa.
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