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ProductsBack/EphA3 (phospho Tyr779) / EphA4 (phospho Tyr779) / EphA5 (phospho Tyr833) antibody
IHC-P analysis of human brain tissue using GTX17348 EphA3 (phospho Tyr779) / EphA4 (phospho Tyr779) / EphA5 (phospho Tyr833) antibody. Left : Primary antibody Right : Primary antibody pre-incubated with the antigen specific peptide
IHC-P analysis of human brain tissue using GTX17348 EphA3 (phospho Tyr779) / EphA4 (phospho Tyr779) / EphA5 (phospho Tyr833) antibody. Left : Primary antibody Right : Primary antibody pre-incubated with the antigen specific peptide
IHC-P analysis of human brain tissue using GTX17348 EphA3 (phospho Tyr779) / EphA4 (phospho Tyr779) / EphA5 (phospho Tyr833) antibody. Left : Primary antibody Right : Primary antibody pre-incubated with the antigen specific peptide

EphA3 (phospho Tyr779) / EphA4 (phospho Tyr779) / EphA5 (phospho Tyr833) antibody

GTX17348
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetEPHA3
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Overview

  • Supplier
    GeneTex
  • Product Name
    EphA3 (phospho Tyr779) / EphA4 (phospho Tyr779) / EphA5 (phospho Tyr833) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:50-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2042
  • Target name
    EPHA3
  • Target description
    EPH receptor A3
  • Target synonyms
    EK4; EPH-like kinase 4; eph-like tyrosine kinase 1; ephrin type-A receptor 3; ETK; ETK1; HEK; HEK4; human embryo kinase 1; testicular tissue protein Li 64; TYRO4; TYRO4 protein tyrosine kinase; tyrosine-protein kinase receptor ETK1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29320
  • Protein Name
    Ephrin type-A receptor 3
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Identification and Therapeutic Intervention of Coactivated Anaplastic Lymphoma Kinase, Fibroblast Growth Factor Receptor 2, and Ephrin Type-A Receptor 5 Kinases in Hepatocellular Carcinoma. Wang X et al., 2019 Feb, Hepatology
    Read more

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Figure 1. Western blot analysis of Eph Receptor A3/EPHA3 using anti-Eph Receptor A3/EPHA3 antibody (A02872-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph Receptor A3/EPHA3 antigen affinity purified polyclonal antibody (Catalog # A02872-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph Receptor A3/EPHA3 at approximately 135 kDa. The expected band size for Eph Receptor A3/EPHA3 is at 110 kDa.
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