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ProductsBack/EPHB1 Antibody (OASG02498)

EPHB1 Antibody (OASG02498)

OASG02498
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetEPHB1
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    EPHB1 Antibody (OASG02498)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Gene ID2047
  • Target name
    EPHB1
  • Target description
    EPH receptor B1
  • Target synonyms
    ELK, EPHT2, Hek6, NET, ephrin type-B receptor 1, EK6, EPH-like kinase 6, eph tyrosine kinase 2, neuronally-expressed EPH-related tyrosine kinase, tyrosine-protein kinase receptor EPH-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Eph receptor B1 using anti-Eph receptor B1 antibody (PB9584). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human Hel whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph receptor B1 antigen affinity purified polyclonal antibody (Catalog # PB9584) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph receptor B1 at approximately 120 kDa. The expected band size for Eph receptor B1 is at 110 kDa.
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