IHC-P analysis of human breast carcinoma tissue using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:50
IHC-P analysis of human breast carcinoma tissue using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:50
ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody
GTX24819
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, C. Elegans, Chicken, Drosophila, Human, Mouse, Rat, Xenopus
TargetMAPK1
Overview
- SupplierGeneTex
- Product NameERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityThe antibody recognizes the same sequence in both proteins (ERK1/2) as long as the T and the Y are phosphorylated. Does not recognize either monophospho- forms of ERK1/2 or the non-phosphorylated proteins.
- Application Supplier NoteWB: 1:1000. IHC-P: 1:10-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID5594
- Target nameMAPK1
- Target descriptionmitogen-activated protein kinase 1
- Target synonymsERK; ERK2; ERK-2; ERT1; extracellular signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAPK 2; MAPK2; mitogen-activated protein kinase 1; mitogen-activated protein kinase 2; NS13; p38; p40; p41; p41mapk; P42MAPK; p42-MAPK; PRKM1; PRKM2; protein tyrosine kinase ERK2
- HostRabbit
- IsotypeIgG
- Protein IDP27361
- Protein NameMitogen-activated protein kinase 3
- Scientific Descriptionkinase involved in intracellular signaling; component of Mapk signaling pathway [RGD, Feb 2006]
- ReactivityBovine, C. Elegans, Chicken, Drosophila, Human, Mouse, Rat, Xenopus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Pancreatic cancer cell-derived semaphorin 3A promotes neuron recruitment to accelerate tumor growth and dissemination.Read more
- Taxifolin attenuates inflammation via suppressing MAPK signal pathway in vitro and in silico analysis.Read more
- Type IV collagen reduces MUC5AC secretion in the lungs of ovalbumin-sensitized mice.Read more
- TRPM7 promotes lipopolysaccharide-induced inflammatory dysfunction in renal tubular epithelial cells. Sun Y et al., 2022 Jul, Immun Inflamm DisRead more
- Integrin beta1 subunit regulates cellular and secreted MUC5AC and MUC5B production in NCI-H292 human lung epithelial cells. Iwashita J et al., 2021 Dec, Biochem Biophys RepRead more
- A novel naphthalimide derivative reduces platelet activation and thrombus formation via suppressing GPVI. Shih TL et al., 2021 Oct, J Cell Mol MedRead more
- Let-7b-3p inhibits tumor growth and metastasis by targeting the BRF2-mediated MAPK/ERK pathway in human lung adenocarcinoma. Li Y et al., 2021 Apr, Transl Lung Cancer ResRead more
- Biological Landscape of Triple Negative Breast Cancers Expressing CTLA-4. Navarrete-Bernal MGC et al., 2020, Front OncolRead more
- Curcumin, demethoxycurcumin, and bisdemethoxycurcumin induced caspase-dependent and -independent apoptosis via Smad or Akt signaling pathways in HOS cells. Huang C et al., 2020 Mar 3, BMC Complement Med TherRead more
- The novel application of cordycepin in maintaining stem cell pluripotency and increasing iPS cell generation efficiency. Wang CH et al., 2020 Feb 10, Sci RepRead more