![ICC/IF analysis of Eca-109 cells using GTX83148 ERK2 antibody [4C11]. Green : ERK2 Red : Actin filament ICC/IF analysis of Eca-109 cells using GTX83148 ERK2 antibody [4C11]. Green : ERK2 Red : Actin filament](https://www.genetex.com/upload/website/prouct_img/normal/GTX83148/GTX83148_20170912_ICCIF_w_23061322_599.webp)
ICC/IF analysis of Eca-109 cells using GTX83148 ERK2 antibody [4C11]. Green : ERK2 Red : Actin filament
ERK2 antibody [4C11]
GTX83148
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMAPK1
Overview
- SupplierGeneTex
- Product NameERK2 antibody [4C11]
- Delivery Days Customer7
- Application Supplier NoteWB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4C11
- ConjugateUnconjugated
- Gene ID5594
- Target nameMAPK1
- Target descriptionmitogen-activated protein kinase 1
- Target synonymsERK, ERK-2, ERK2, ERT1, MAPK2, NS13, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, p42-MAPK, mitogen-activated protein kinase 1, MAP kinase 1, MAP kinase 2, MAPK 2, extracellular signal-regulated kinase 2, mitogen-activated protein kinase 2, protein tyrosine kinase ERK2
- HostMouse
- IsotypeIgG2a
- Protein IDP28482
- Protein NameMitogen-activated protein kinase 1
- Scientific DescriptionThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jul 2008]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![IHC-P analysis of human lung carcinoma (left) and breast carcinoma (right) using GTX83148 ERK2 antibody [4C11]. IHC-P analysis of human lung carcinoma (left) and breast carcinoma (right) using GTX83148 ERK2 antibody [4C11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83148/GTX83148_20170912_IHC-P_w_23061322_671.webp)
![WB analysis of HeLa (1), NIH3T3 (2), MCF-7 (3), HEK293 (4), Jurkat (5), A549 (6), NTERA-2 (7) and SMMC-7721 (8) cell lysate using GTX83148 ERK2 antibody [4C11]. WB analysis of HeLa (1), NIH3T3 (2), MCF-7 (3), HEK293 (4), Jurkat (5), A549 (6), NTERA-2 (7) and SMMC-7721 (8) cell lysate using GTX83148 ERK2 antibody [4C11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83148/GTX83148_20170912_WB_w_23061322_402.webp)


![WB analysis of wild-type (WT) and ERK2 knockout (KO) HeLa cell extracts using GTX00945 ERK2 antibody [GT1183]. Dilution : 1:1000 Laoding : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00945/GTX00945_20200327_WB_1_w_23053121_922.webp)
![IHC-P analysis of human pancreas tissue using GTX84151 ERK2 antibody [4C2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84151/GTX84151_2379_IHC-P_w_23061420_981.webp)
![IP analysis of DDDDK tagged ERK2 overexpressed HEK293T lysate using GTX84153 ERK2 antibody [4D10].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX84153/GTX84153_3348_IP_w_23061420_970.webp)
![IHC-P analysis of human breast adenocarcinoma tissue using GTX84154 ERK2 antibody [6F8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84154/GTX84154_2380_IHC-P_w_23061420_118.webp)
![IHC-P analysis of breast adenocarcinoma tissue using GTX84159 ERK2 antibody [6E5]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84159/GTX84159_2382_IHC-P_w_23061420_965.webp)