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IP analysis of DDDDK tagged ERK2 overexpressed HEK293T lysate using GTX84153 ERK2 antibody [4D10].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate
IP analysis of DDDDK tagged ERK2 overexpressed HEK293T lysate using GTX84153 ERK2 antibody [4D10].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate
WB analysis of wild type and ERK2 knock out HeLa cell lysate(10microg per lane) using GTX84153 ERK2 antibody [4D10]. Dilution : 1:200
WB analysis of HEK293T cells transfected with ERK2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84153 ERK2 antibody [4D10]. Loading : 5 ug per lane
WB analysis of various cell lines using GTX84153 ERK2 antibody [4D10]. Loading : 35 ug per lane
IP analysis of DDDDK tagged ERK2 overexpressed HEK293T lysate using GTX84153 ERK2 antibody [4D10].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate

ERK2 antibody [4D10]

Research Use Only
GTX84153
GeneTex
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityCanine, Human, Monkey, Rat
TargetMAPK1
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Overview

  • Supplier
    GeneTex
  • Product Name
    ERK2 antibody [4D10] - KO/KD, Overexpression Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:2000. IP: 2-4ug/mg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4D10
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Formulation
    Liquid
  • Gene ID5594
  • Target name
    MAPK1
  • Target description
    mitogen-activated protein kinase 1
  • Target synonyms
    ERK; ERK2; ERK-2; ERT1; extracellular signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAPK 2; MAPK2; mitogen-activated protein kinase 1; mitogen-activated protein kinase 2; NS13; p38; p40; p41; p41mapk; P42MAPK; p42-MAPK; PRKM1; PRKM2; protein tyrosine kinase ERK2
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP28482
  • Protein Name
    Mitogen-activated protein kinase 1
  • Scientific Description
    Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1 - required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2. Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • Reactivity
    Canine, Human, Monkey, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203