IP analysis of DDDDK tagged ERK2 overexpressed HEK293T lysate using GTX84153 ERK2 antibody [4D10].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate
![WB analysis of wild type and ERK2 knock out HeLa cell lysate(10microg per lane) using GTX84153 ERK2 antibody [4D10]. Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX84153/GTX84153_4154_20181228_WB_w_23061420_596.webp "WB analysis of wild type and ERK2 knock out HeLa cell lysate(10microg per lane) using GTX84153 ERK2 antibody [4D10]. Dilution : 1:200")
![WB analysis of HEK293T cells transfected with ERK2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84153 ERK2 antibody [4D10]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84153/GTX84153_4155_WB_w_23061420_796.webp "WB analysis of HEK293T cells transfected with ERK2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84153 ERK2 antibody [4D10]. Loading : 5 ug per lane")
![WB analysis of various cell lines using GTX84153 ERK2 antibody [4D10]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84153/GTX84153_4156_WB_w_23061420_383.webp "WB analysis of various cell lines using GTX84153 ERK2 antibody [4D10]. Loading : 35 ug per lane")
IP analysis of DDDDK tagged ERK2 overexpressed HEK293T lysate using GTX84153 ERK2 antibody [4D10].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate
ERK2 antibody [4D10] - KO/KD, Overexpression Validated
GTX84153
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityCanine, Human, Monkey, Rat
TargetMAPK1
Overview
- SupplierGeneTex
- Product NameERK2 antibody [4D10] - KO/KD, Overexpression Validated
- Delivery Days Customer9
- ApplicationsImmunoPrecipitation, Western Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4D10
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5594
- Target nameMAPK1
- Target descriptionmitogen-activated protein kinase 1
- Target synonymsERK; ERK2; ERK-2; ERT1; extracellular signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAPK 2; MAPK2; mitogen-activated protein kinase 1; mitogen-activated protein kinase 2; NS13; p38; p40; p41; p41mapk; P42MAPK; p42-MAPK; PRKM1; PRKM2; protein tyrosine kinase ERK2
- HostMouse
- IsotypeIgG2a
- Scientific DescriptionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1 - required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2. Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
- ReactivityCanine, Human, Monkey, Rat
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203