WB (peptide competition) analysis of HEK293 cells transiently transfected with plasmids expressing ERK5 kinase domain (ERK5kin) and constitutively activated MEK5D-D using GTX25686 ERK5 (phospho Thr218/Tyr220) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 3), a generic phosphothreonine-containing peptide (Lane 4), a generic phosphotyrosine-containing peptide (Lane 5), or the phosphopeptide immunogen (Lane 6 ). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
WB (peptide competition) analysis of HEK293 cells transiently transfected with plasmids expressing ERK5 kinase domain (ERK5kin) and constitutively activated MEK5D-D using GTX25686 ERK5 (phospho Thr218/Tyr220) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 3), a generic phosphothreonine-containing peptide (Lane 4), a generic phosphotyrosine-containing peptide (Lane 5), or the phosphopeptide immunogen (Lane 6 ). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
ERK5 (phospho Thr218/Tyr220) antibody
GTX25686
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Primate
TargetMAPK7
Overview
- SupplierGeneTex
- Product NameERK5 (phospho Thr218/Tyr220) antibody - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificitySome cross-reactivity is observed with endogenous ERK1 and 2 (44 and 42 kDa, respectively) due to the high levels of expression and activation of this protein typically observed with most cell types.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID5598
- Target nameMAPK7
- Target descriptionmitogen-activated protein kinase 7
- Target synonymsbig MAP kinase 1; BMK1; BMK-1; BMK1 kinase; ERK4; ERK5; ERK-5; extracellular-signal-regulated kinase 5; MAP kinase 7; MAPK 7; mitogen-activated protein kinase 7; PRKM7
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Protein IDQ13164
- Protein NameMitogen-activated protein kinase 7
- Scientific DescriptionThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Primate
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203