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ProductsBack/ERp57 (PDIA3) Goat Polyclonal Antibody
Endogenous ERP57- endoplasmic reticulum lumen marker detected at 1/500 dilution; lysates at 100 ug per lane and rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
Endogenous ERP57- endoplasmic reticulum lumen marker detected at 1/500 dilution; lysates at 100 ug per lane and rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
Endogenous ERP57- endoplasmic reticulum lumen marker detected at 1/500 dilution; lysates at 100 ug per lane and rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

ERp57 (PDIA3) Goat Polyclonal Antibody

AB0004-500
OriGene
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetPDIA3
1.5 mg
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Overview

  • Supplier
    OriGene
  • Product Name
    ERp57 (PDIA3) Goat Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID2923
  • Target name
    PDIA3
  • Target description
    protein disulfide isomerase family A member 3
  • Target synonyms
    ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HEL-S-269, HEL-S-93n, HsT17083, P58, PI-PLC, protein disulfide-isomerase A3, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, ER protein 57, ER protein 60, disulfide isomerase ER-60, endoplasmic reticulum P58, endoplasmic reticulum resident protein 57, endoplasmic reticulum resident protein 60, epididymis secretory protein Li 269, epididymis secretory sperm binding protein Li 93n, glucose regulated protein, 58kDa, phospholipase C-alpha, protein disulfide isomerase-associated 3
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDP30101
  • Protein Name
    Protein disulfide-isomerase A3
  • Scientific Description
    Goat Polyclonal Anti-ERP57 Antibody
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of ERp57 using anti-ERp57 antibody (PB9772). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate, Lane 2: Mouse Liver Tissue Lysate, Lane 3: A549 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERp57 antigen affinity purified polyclonal antibody (Catalog # PB9772) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERp57 at approximately 57KD. The expected band size for ERp57 is at 57KD.
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