Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Estrogen Receptor alpha antibody [HL1082] (GTX636203) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [HL1082] (GTX636203) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636203/GTX636203_44382_20210730_WB_B_w_23061202_936.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [HL1082] (GTX636203) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [HL1082] (GTX636203) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636203/GTX636203_44382_20210723_WB_w_23061202_713.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [HL1082] (GTX636203) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Estrogen Receptor alpha antibody [HL1082] detects Estrogen Receptor alpha protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Estrogen Receptor alpha stained by Estrogen Receptor alpha antibody [HL1082] (GTX636203) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636203/GTX636203_44382_20210813_IHC-P_w_23061202_287.webp "Estrogen Receptor alpha antibody [HL1082] detects Estrogen Receptor alpha protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Estrogen Receptor alpha stained by Estrogen Receptor alpha antibody [HL1082] (GTX636203) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Estrogen Receptor alpha antibody [HL1082] (GTX636203) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. *The competitor is not affiliated with GeneTex and does not endorse this product.
Estrogen Receptor alpha antibody [HL1082]
GTX636203
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetESR1
Overview
- SupplierGeneTex
- Product NameEstrogen Receptor alpha antibody [HL1082]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1082
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2099
- Target nameESR1
- Target descriptionestrogen receptor 1
- Target synonymsER, ESR, ESRA, ESTRR, Era, NR3A1, estrogen receptor, E2 receptor alpha, ER-alpha, estradiol receptor, estrogen nuclear receptor alpha, estrogen receptor alpha E1-E2-1-2, estrogen receptor alpha E1-N2-E2-1-2, nuclear receptor subfamily 3 group A member 1, oestrogen receptor alpha
- HostRabbit
- IsotypeIgG
- Protein IDP03372
- Protein NameEstrogen receptor
- Scientific DescriptionThis gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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