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ProductsBack/Estrogen Receptor beta antibody [6A12]

Estrogen Receptor beta antibody [6A12]

GTX70178
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
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Product group Antibodies
TargetESR2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Estrogen Receptor beta antibody [6A12]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 5 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6A12
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2100
  • Target name
    ESR2
  • Target description
    estrogen receptor 2
  • Target synonyms
    Erb; ER-BETA; ESRB; ESR-BETA; ESTRB; estrogen receptor beta; estrogen receptor beta 4; estrogen receptor beta splice variant, ERbeta2delta7; estrogen receptor beta splice variant, ERbeta4delta7; estrogen receptor beta splice variant, ERbeta6; estrogen receptor beta splice variant, ERbeta6delta7; estrogen receptor beta splice variant, ERbeta7; estrogen receptor beta splice variant, ERbeta7delta7; estrogen receptor beta splice variant, ERbetaEx. 4L; estrogen receptor beta splice variant, ERbetaEx. 6L; NR3A2; nuclear receptor subfamily 3 group A member 2; ODG8; oestrogen receptor beta
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ92731
  • Protein Name
    Estrogen receptor beta
  • Scientific Description
    This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Estrogen receptor beta causes a G2 cell cycle arrest by inhibiting CDK1 activity through the regulation of cyclin B1, GADD45A, and BTG2. Paruthiyil S et al., 2011 Oct, Breast Cancer Res Treat
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  • Estrogen receptor beta binds to and regulates three distinct classes of target genes. Vivar OI et al., 2010 Jul 16, J Biol Chem
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  • Selective activation of estrogen receptor-beta target genes by 3,3-diindolylmethane. Vivar OI et al., 2010 Apr, Endocrinology
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  • Activity and intracellular location of estrogen receptors alpha and beta in human bronchial epithelial cells. Ivanova MM et al., 2009 Jun 16, Mol Cell Endocrinol
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  • Resveratrol stimulates nitric oxide production by increasing estrogen receptor alpha-Src-caveolin-1 interaction and phosphorylation in human umbilical vein endothelial cells. Klinge CM et al., 2008 Jul, FASEB J
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  • Selective activation of estrogen receptor-beta transcriptional pathways by an herbal extract. Cvoro A et al., 2007 Feb, Endocrinology
    Read more
  • Distinct roles of unliganded and liganded estrogen receptors in transcriptional repression. Cvoro A et al., 2006 Feb 17, Mol Cell
    Read more
  • Estradiol and selective estrogen receptor modulators differentially regulate target genes with estrogen receptors alpha and beta. Tee MK et al., 2004 Mar, Mol Biol Cell
    Read more

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Figure 1. Western blot analysis of ESR2 using anti-ESR2 antibody (A00786-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESR2 antigen affinity purified polyclonal antibody (Catalog # A00786-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESR2 at approximately 59 kDa. The expected band size for ESR2 is at 59 kDa.
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