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Estrogen Receptor beta antibody [7B10.7]

GTX70182
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation
Product group Antibodies
TargetESR2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Estrogen Receptor beta antibody [7B10.7]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 5 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    7B10.7
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2100
  • Target name
    ESR2
  • Target description
    estrogen receptor 2
  • Target synonyms
    Erb; ER-BETA; ESRB; ESR-BETA; ESTRB; estrogen receptor beta; estrogen receptor beta 4; estrogen receptor beta splice variant, ERbeta2delta7; estrogen receptor beta splice variant, ERbeta4delta7; estrogen receptor beta splice variant, ERbeta6; estrogen receptor beta splice variant, ERbeta6delta7; estrogen receptor beta splice variant, ERbeta7; estrogen receptor beta splice variant, ERbeta7delta7; estrogen receptor beta splice variant, ERbetaEx. 4L; estrogen receptor beta splice variant, ERbetaEx. 6L; NR3A2; nuclear receptor subfamily 3 group A member 2; ODG8; oestrogen receptor beta
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ92731
  • Protein Name
    Estrogen receptor beta
  • Scientific Description
    This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Insights into the Role of Estrogen Receptor beta in Triple-Negative Breast Cancer. Sellitto A et al., 2020 Jun 5, Cancers (Basel)
    Read more
  • Estrogen Receptor-beta Modulation of the ERalpha-p53 Loop Regulating Gene Expression, Proliferation, and Apoptosis in Breast Cancer. Lu W et al., 2017 Aug, Horm Cancer
    Read more
  • Comprehensive assessment of estrogen receptor beta antibodies in cancer cell line models and tissue reveals critical limitations in reagent specificity. Nelson AW et al., 2017 Jan 15, Mol Cell Endocrinol
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  • Estrogen receptor beta regulates endometriotic cell survival through serum and glucocorticoid-regulated kinase activation. Monsivais D et al., 2016 May, Fertil Steril
    Read more
  • ERbeta- and prostaglandin E2-regulated pathways integrate cell proliferation via Ras-like and estrogen-regulated growth inhibitor in endometriosis. Monsivais D et al., 2014 Aug, Mol Endocrinol
    Read more
  • Preferential estrogen receptor beta ligands reduce Bcl-2 expression in hormone-resistant breast cancer cells to increase autophagy. Ruddy SC et al., 2014 Jul, Mol Cancer Ther
    Read more
  • Mechanisms enforcing the estrogen receptor beta selectivity of botanical estrogens. Jiang Y et al., 2013 Nov, FASEB J
    Read more
  • Integrative genomics of gene and metabolic regulation by estrogen receptors alpha and beta, and their coregulators. Madak-Erdogan Z et al., 2013 Jun 18, Mol Syst Biol
    Read more
  • Induction of quinone reductase by tamoxifen or DPN protects against mammary tumorigenesis. Krishnamurthy N et al., 2012 Oct, FASEB J
    Read more
  • Estrogen receptor beta causes a G2 cell cycle arrest by inhibiting CDK1 activity through the regulation of cyclin B1, GADD45A, and BTG2. Paruthiyil S et al., 2011 Oct, Breast Cancer Res Treat
    Read more

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Figure 1. Western blot analysis of ESR2 using anti-ESR2 antibody (A00786-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESR2 antigen affinity purified polyclonal antibody (Catalog # A00786-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESR2 at approximately 59 kDa. The expected band size for ESR2 is at 59 kDa.
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