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IHC-P analysis of T-Cell lymphoma tissue using GTX01878 EZH2 antibody [6A10]. Note nuclear staining.
IHC-P analysis of T-Cell lymphoma tissue using GTX01878 EZH2 antibody [6A10]. Note nuclear staining.
IHC-P analysis of T-Cell lymphoma tissue using GTX01878 EZH2 antibody [6A10]. Note nuclear staining.

EZH2 antibody [6A10]

GTX01878
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetEZH2
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Overview

  • Supplier
    GeneTex
  • Product Name
    EZH2 antibody [6A10]
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6A10
  • Conjugate
    Unconjugated
  • Gene ID2146
  • Target name
    EZH2
  • Target description
    enhancer of zeste 2 polycomb repressive complex 2 subunit
  • Target synonyms
    ENX-1, ENX1, EZH2b, KMT6, KMT6A, WVS, WVS2, histone-lysine N-methyltransferase EZH2, enhancer of zeste homolog 2, lysine N-methyltransferase 6
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ15910
  • Protein Name
    Histone-lysine N-methyltransferase EZH2
  • Scientific Description
    This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KMT6/EZH2 using anti-KMT6/EZH2 antibody (A00050-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KMT6/EZH2 antigen affinity purified polyclonal antibody (Catalog # A00050-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KMT6/EZH2 at approximately 98 kDa. The expected band size for KMT6/EZH2 is at 85 kDa.
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