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Western Blot analysis of 293T and K562 cell, Human fetal kidney tissue using F2R Polyclonal Antibody at dilution of 1:650
Western Blot analysis of 293T and K562 cell, Human fetal kidney tissue using F2R Polyclonal Antibody at dilution of 1:650
Western Blot analysis of 293T and K562 cell, Human fetal kidney tissue using F2R Polyclonal Antibody at dilution of 1:650

F2R Polyclonal Antibody

E-AB-15818
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    F2R Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.3mg/ml
  • Scientific Description
    Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of F2R using anti-F2R antibody (A03352-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-F2R antigen affinity purified polyclonal antibody (Catalog # A03352-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for F2R at approximately 47KD. The expected band size for F2R is at 47KD.
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