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Immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using F3 Polyclonal Antibody at dilution of 1:25
Immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using F3 Polyclonal Antibody at dilution of 1:25
Immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using F3 Polyclonal Antibody at dilution of 1:25

F3 Polyclonal Antibody

E-AB-10636
Elabscience
ApplicationsWestern Blot
Product group Antibodies
TargetF3
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Overview

  • Supplier
    Elabscience
  • Product Name
    F3 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2152
  • Target name
    F3
  • Target description
    coagulation factor III, tissue factor
  • Target synonyms
    CD142, TF, TFA, tissue factor, coagulation factor III (thromboplastin, tissue factor)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13726
  • Protein Name
    Tissue factor
  • Scientific Description
    This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Tissue Factor using anti-Tissue Factor antibody (PB9701). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tissue Factor antigen affinity purified polyclonal antibody (Catalog # PB9701) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tissue Factor at approximately 50 kDa. The expected band size for Tissue Factor is at 33 kDa.
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