WB analysis of HeLa cells treated with 100 ng/mL Taxol for 24 hours using GTX24790 FAK (phospho Ser722) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphoserine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.
WB analysis of HeLa cells treated with 100 ng/mL Taxol for 24 hours using GTX24790 FAK (phospho Ser722) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphoserine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.
FAK (phospho Ser722) antibody
GTX24790
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetPTK2
Overview
- SupplierGeneTex
- Product NameFAK (phospho Ser722) antibody - Orthogonal Validated
- Delivery Days Customer9
- ApplicationsImmunoPrecipitation, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID5747
- Target namePTK2
- Target descriptionprotein tyrosine kinase 2
- Target synonymsFADK; FADK 1; FAK; FAK1; FAK-related non-kinase polypeptide; focal adhesion kinase 1; focal adhesion kinase-related nonkinase; FRNK; p125FAK; pp125FAK; PPP1R71; protein phosphatase 1 regulatory subunit 71; PTK2 protein tyrosine kinase 2
- HostRabbit
- IsotypeIgG
- Protein IDQ05397
- Protein NameFocal adhesion kinase 1
- Scientific DescriptionThis gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203