WB (peptide competition) analysis of 3T3-L1 cells stimulated with 50 ng/ml LIF for 15 minutes (Lane 2-4) using GTX24804 FAK (phospho Tyr861) antibody prior incubated with the phosphopeptide immunogen (Lane 1), or genertic phosphopeptide (Lane 2), the non-phosphopeptide corresponding to generic phosphoserine-containing peptide (Lane 3), or no peptide (Lane 4).. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
WB (peptide competition) analysis of 3T3-L1 cells stimulated with 50 ng/ml LIF for 15 minutes (Lane 2-4) using GTX24804 FAK (phospho Tyr861) antibody prior incubated with the phosphopeptide immunogen (Lane 1), or genertic phosphopeptide (Lane 2), the non-phosphopeptide corresponding to generic phosphoserine-containing peptide (Lane 3), or no peptide (Lane 4).. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
FAK (phospho Tyr861) antibody
GTX24804
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityAmphibian, Canine, Chicken, Hamster, Human, Mouse, Rat, Xenopus, Zebra Fish
TargetPTK2
Overview
- SupplierGeneTex
- Product NameFAK (phospho Tyr861) antibody - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. ICC/IF: 1 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID5747
- Target namePTK2
- Target descriptionprotein tyrosine kinase 2
- Target synonymsFADK; FADK 1; FAK; FAK1; FAK-related non-kinase polypeptide; focal adhesion kinase 1; focal adhesion kinase-related nonkinase; FRNK; p125FAK; pp125FAK; PPP1R71; protein phosphatase 1 regulatory subunit 71; PTK2 protein tyrosine kinase 2
- HostRabbit
- IsotypeIgG
- Protein IDQ05397
- Protein NameFocal adhesion kinase 1
- Scientific DescriptionThis gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]
- ReactivityAmphibian, Canine, Chicken, Hamster, Human, Mouse, Rat, Xenopus, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203