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ProductsBack/FGF2 Mouse Monoclonal Antibody [Clone ID: 2H5G2C11]
Western blot analysis using FGF2 mouse mAb against truncated FGF2 recombinant protein.
Western blot analysis using FGF2 mouse mAb against truncated FGF2 recombinant protein.
Western blot analysis using FGF2 mouse mAb against truncated FGF2 recombinant protein.

FGF2 Mouse Monoclonal Antibody [Clone ID: 2H5G2C11]

AM06116SU-N
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFGF2
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    FGF2 Mouse Monoclonal Antibody [Clone ID: 2H5G2C11]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2H5G2C11
  • Gene ID2247
  • Target name
    FGF2
  • Target description
    fibroblast growth factor 2
  • Target synonyms
    BFGF, FGF-2, FGFB, HBGF-2, fibroblast growth factor 2, basic fibroblast growth factor bFGF, fibroblast growth factor 2 (basic), heparin-binding growth factor 2, prostatropin
  • Host
    Mouse
  • Isotype
    IgG
  • Protein IDP09038
  • Protein Name
    Fibroblast growth factor 2
  • Scientific Description
    FGF2 mouse monoclonal antibody, clone 2H5G2C11, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of FGF2 using anti-FGF2 antibody (A00121-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SKOV3 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (Catalog # A00121-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF2 at approximately 17-21 kDa. The expected band size for FGF2 is at 31 kDa.
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