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Fibronectin antibody

GTX72724
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetFN1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Fibronectin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    Immunohistochemistry (IHC) 1:100-1:200 for 30 minute at room temperature using polymer system or streptavidin-biotin system. Formalin-fixed paraffin-embedded (FFPE) tissue sections and cell smears require antigen retriever, boiling tissue in 10 mM citrate buffer, pH 6.0 for 10-20 minute, followed by cooling at room temperature for 20 minutes.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2335
  • Target name
    FN1
  • Target description
    fibronectin 1
  • Target synonyms
    CIG; cold-insoluble globulin; ED-B; epididymis secretory sperm binding protein; fibronectin; FINC; FN; FNZ; GFND; GFND2; LETS; migration-stimulating factor; MSF; SMDCF
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02751
  • Protein Name
    Fibronectin
  • Scientific Description
    This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Large-scale isolation and cytotoxicity of extracellular vesicles derived from activated human natural killer cells. Jong AY et al., 2017, J Extracell Vesicles
    Read more
  • Enhanced hepatic differentiation of rat bone marrow-derived mesenchymal stem cells in spheroidal aggregate culture on a decellularized liver scaffold. Bao J et al., 2016 Aug, Int J Mol Med
    Read more
  • Hemocompatibility improvement of perfusion-decellularized clinical-scale liver scaffold through heparin immobilization. Bao J et al., 2015 Jun 1, Sci Rep
    Read more
  • Optimizing perfusion-decellularization methods of porcine livers for clinical-scale whole-organ bioengineering. Wu Q et al., 2015, Biomed Res Int
    Read more

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Figure 1. Western blot analysis of FN1 using anti-FN1 antibody (A00564-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 3: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FN1 antigen affinity purified polyclonal antibody (Catalog # A00564-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FN1 at approximately 220-400 kDa. The expected band size for FN1 is at 272 kDa.
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