
FIS1 antibody detects FIS1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: FIS1 protein stained by FIS1 antibody (GTX630983) diluted at 1:1000. Red: MitoTrackerR Red CMXRos, a mitochondria tracker. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
FIS1 antibody [GT4211]
GTX630983
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetFIS1
Overview
- SupplierGeneTex
- Product NameFIS1 antibody [GT4211]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT4211
- Concentration2.95 mg/ml
- ConjugateUnconjugated
- Gene ID51024
- Target nameFIS1
- Target descriptionfission, mitochondrial 1
- Target synonymsCGI-135, TTC11, mitochondrial fission 1 protein, FIS1 homolog, H_NH0132A01.6, TPR repeat protein 11, fission 1 (mitochondrial outer membrane) homolog, hFis1, mitochondrial fission molecule, tetratricopeptide repeat domain 11, tetratricopeptide repeat protein 11
- HostMouse
- IsotypeIgG2b
- Protein IDQ9Y3D6
- Protein NameMitochondrial fission 1 protein
- Scientific DescriptionThe balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM]
- ReactivityHuman, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [GT4211] (GTX630983) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [GT4211] (GTX630983) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630983/GTX630983_41904_20191004_WB_R_w_23061202_888.webp)

![Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [GT4211] (GTX630983) diluted at 1:500. Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [GT4211] (GTX630983) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630983/GTX630983_41904_20161013_WB_shRNA_watermark_w_23061202_286.webp)
![FIS1 antibody [GT4211] detects FIS1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature. Green: FIS1 protein stained by FIS1 antibody [GT4211] (GTX630983) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm. FIS1 antibody [GT4211] detects FIS1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature. Green: FIS1 protein stained by FIS1 antibody [GT4211] (GTX630983) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630983/GTX630983_41904_20150821_IFA_w_23061202_538.webp)

![IHC-P analysis of rat brain tissue section using GTX00950 FIS1 antibody [GT1188]. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00950/GTX00950_20200327_IHC-P_18_w_23053121_953.webp)


![FIS1 antibody [GT12112] detects FIS1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15 % SDS-PAGE, and blotted with FIS1 antibody [GT12112] (GTX630992) diluted by 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX630992/GTX630992_41750_WB_w_23061202_870.webp)
![Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [GT9810] (GTX631209) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631209/GTX631209_41743_20191004_WB_R_w_23061202_369.webp)


