Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FLI1 antibody [HL1805] (GTX637457) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FLI1 antibody [HL1805] (GTX637457) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637457/GTX637457_44970_20230303_WB_TPM_watermark_23030717_645.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FLI1 antibody [HL1805] (GTX637457) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Immunoprecipitation of FLI1 protein from Raji whole cell extract using 5 μg of FLI1 antibody [HL1805] (GTX637457). Western blot analysis was performed using FLI1 antibody [HL1805] (GTX637457). EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).](https://www.genetex.com/upload/website/prouct_img/normal/GTX637457/GTX637457_44970_20240419_IP_24042400_126.webp "Immunoprecipitation of FLI1 protein from Raji whole cell extract using 5 μg of FLI1 antibody [HL1805] (GTX637457). Western blot analysis was performed using FLI1 antibody [HL1805] (GTX637457). EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).")
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FLI1 antibody [HL1805] (GTX637457) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
FLI1 antibody [HL1805]
GTX637457
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetFLI1
Overview
- SupplierGeneTex
- Product NameFLI1 antibody [HL1805]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:5000-1:100000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1805
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2313
- Target nameFLI1
- Target descriptionFli-1 proto-oncogene, ETS transcription factor
- Target synonymsBDPLT21, EWSR2, FLI-1, SIC-1, Friend leukemia integration 1 transcription factor, Ewing sarcoma breakpoint region 2, Friend leukemia virus integration 1, transcription factor ERGB
- HostRabbit
- IsotypeIgG
- Protein IDQ01543
- Protein NameFriend leukemia integration 1 transcription factor
- Scientific DescriptionThis gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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