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Immunocytochemical staining of FLIP in HeLa cells with FLIP antibody (GTX28421) at 5microg/ml.
Immunocytochemical staining of FLIP in HeLa cells with FLIP antibody (GTX28421) at 5microg/ml.
Immunocytochemical staining of FLIP in HeLa cells with FLIP antibody (GTX28421) at 5microg/ml.

FLIP antibody

GTX28421
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCFLAR
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Overview

  • Supplier
    GeneTex
  • Product Name
    FLIP antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1-2microg/ml. ICC/IF: 5microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8837
  • Target name
    CFLAR
  • Target description
    CASP8 and FADD like apoptosis regulator
  • Target synonyms
    CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS, cFLIP, CASP8 and FADD-like apoptosis regulator, FADD-like antiapoptotic molecule 1, MACH-related inducer of toxicity, caspase homolog, caspase-eight-related protein, caspase-like apoptosis regulatory protein, caspase-related inducer of apoptosis, cellular FLICE-like inhibitory protein, inhibitor of FLICE, testis secretory sperm-binding protein Li 233m, usurpin beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15519
  • Protein Name
    CASP8 and FADD-like apoptosis regulator
  • Scientific Description
    The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Ovadje P, Ammar S, Guerrero JA, et al. Dandelion root extract affects colorectal cancer proliferation and survival through the activation of multiple death signalling pathways. Oncotarget. 2016,7(45):73080-73100. doi: 10.18632/oncotarget.11485
    Read this paper

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Figure 1. Western blot analysis of FLIP using anti-FLIP antibody (A01295-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human PANC-1 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human MDA-MB-231 whole cell lysates, Lane 7: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLIP antigen affinity purified polyclonal antibody (Catalog # A01295-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLIP at approximately 43KD. The expected band size for FLIP is at 55KD.
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