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WB analysis of various sample lysates using GTX03466 FNDC5 antibody. Lane 1 : rat heart tissue lysate Lane 2 : mouse heart tissue lysate Dilution : 0.5 microg/ml Loading : 50microg per lane
WB analysis of various sample lysates using GTX03466 FNDC5 antibody. Lane 1 : rat heart tissue lysate Lane 2 : mouse heart tissue lysate Dilution : 0.5 microg/ml Loading : 50microg per lane
WB analysis of various sample lysates using GTX03466 FNDC5 antibody. Lane 1 : rat heart tissue lysate Lane 2 : mouse heart tissue lysate Dilution : 0.5 microg/ml Loading : 50microg per lane

FNDC5 antibody

GTX03466
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetFNDC5
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Overview

  • Supplier
    GeneTex
  • Product Name
    FNDC5 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.5microg/ml. ELISA: 0.1-0.5microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID252995
  • Target name
    FNDC5
  • Target description
    fibronectin type III domain containing 5
  • Target synonyms
    FRCP2, irisin, fibronectin type III domain-containing protein 5, fibronectin type III repeat-containing protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NAU1
  • Protein Name
    Fibronectin type III domain-containing protein 5
  • Scientific Description
    This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FNDC5 using anti-FNDC5 antibody (A02538-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FNDC5 antigen affinity purified polyclonal antibody (Catalog # A02538-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FNDC5 at approximately 23KD. The expected band size for FNDC5 is at 23KD.
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