Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FOX3/NeuN Polyclonal Antibody, Unconjugated (bs-1613R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FOX3/NeuN Polyclonal Antibody, Unconjugated (bs-1613R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
NeuN Polyclonal Antibody
BS-1613R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetRBFOX3
Overview
- SupplierBioss
- Product NameNeuN Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID146713
- Target nameRBFOX3
- Target descriptionRNA binding fox-1 homolog 3
- Target synonymsFOX-3, FOX3, HRNBP3, NEUN, RNA binding protein fox-1 homolog 3, RNA binding protein, fox-1 homolog 3, fox-1 homolog C, hexaribonucleotide binding protein 3, neuN antigen, neuronal nuclei antigen
- HostRabbit
- IsotypeIgG
- Protein IDA6NFN3
- Protein NameRNA binding protein fox-1 homolog 3
- Storage Instruction-20°C
- UNSPSC12352203
References
- Chen YC, Chiu YJ, Lin CH, et al. Indole Compound NC009-1 Augments APOE and TRKA in Alzheimer's Disease Cell and Mouse Models for Neuroprotection and Cognitive Improvement. J Alzheimers Dis. 2019,67(2):737-756. doi: 10.3233/JAD-180643Read this paper
- Wang J, Tian W, Wang S, et al. Anti-inflammatory and retinal protective effects of capsaicin on ischaemia-induced injuries through the release of endogenous somatostatin. Clin Exp Pharmacol Physiol. 2017,44(7):803-814. doi: 10.1111/1440-1681.12769Read this paper
- Kopec AM, Rivera PD, Lacagnina MJ, et al. Optimized solubilization of TRIzol-precipitated protein permits Western blotting analysis to maximize data available from brain tissue. J Neurosci Methods. 2017,280:64-76. doi: 10.1016/j.jneumeth.2017.02.002Read this paper
- Hu HX, Lin RH, Zhu XQ, et al. [Corrigendum] Anti-inflammatory effects of Gualou Guizhi decoction in transient focal cerebral ischemic brains. Mol Med Rep. 2015,12(3):3998. doi: 10.3892/mmr.2015.3893Read this paper
- Guo SL, Zhang ZY, Xu Y, et al. Bone marrow-derived, neural-like cells have the characteristics of neurons to protect the peripheral nerve in microenvironment. Stem Cells Int. 2015,2015:941625. doi: 10.1155/2015/941625Read this paper
- Wei G, Chen YB, Chen DF, et al. β-Asarone inhibits neuronal apoptosis via the CaMKII/CREB/Bcl-2 signaling pathway in an in vitro model and AβPP/PS1 mice. J Alzheimers Dis. 2013,33(3):863-80. doi: 10.3233/JAD-2012-120865Read this paper
Datasheet
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