
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FOX3/NeuN Polyclonal Antibody, Unconjugated (bs-1613R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
NeuN Polyclonal Antibody
BS-1613R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Rat
TargetRBFOX3
Overview
- SupplierBioss
- Product NameNeuN Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID146713
- Target nameRBFOX3
- Target descriptionRNA binding fox-1 homolog 3
- Target synonymsFOX-3, FOX3, HRNBP3, NEUN, RNA binding protein fox-1 homolog 3, RNA binding protein, fox-1 homolog 3, fox-1 homolog C, hexaribonucleotide binding protein 3, neuN antigen, neuronal nuclei antigen
- HostRabbit
- IsotypeIgG
- Protein IDA6NFN3
- Protein NameRNA binding protein fox-1 homolog 3
- ReactivityBovine, Canine, Equine, Human, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC41116161
References
- Indole Compound NC009-1 Augments APOE and TRKA in Alzheimers Disease Cell and Mouse Models for Neuroprotection and Cognitive Improvement. Chen YC et al., 2019, J Alzheimers DisRead this paper
- Anti-inflammatory and retinal protective effects of capsaicin on ischaemia-induced injuries through the release of endogenous somatostatin. Wang J et al., 2017 Jul, Clin Exp Pharmacol PhysiolRead this paper
- Optimized solubilization of TRIzol-precipitated protein permits Western blotting analysis to maximize data available from brain tissue. Kopec AM et al., 2017 Mar 15, J Neurosci MethodsRead this paper
- Anti-inflammatory effects of Gualou Guizhi decoction in transient focal cerebral ischemic brains. [Corrected]. Hu HX et al., 2015 Sep, Mol Med RepRead this paper
- Death of Neurons following Injury Requires Conductive Neuronal Gap Junction Channels but Not a Specific Connexin. Fontes JD et al., 2015, PLoS OneRead this paper
- Bone marrow-derived, neural-like cells have the characteristics of neurons to protect the peripheral nerve in microenvironment. Guo SL et al., 2015, Stem Cells IntRead this paper
- beta-Asarone inhibits neuronal apoptosis via the CaMKII/CREB/Bcl-2 signaling pathway in an in vitro model and AbetaPP/PS1 mice. Wei G et al., 2013, J Alzheimers DisRead this paper











![NeuN antibody detects NeuN protein at nucleus by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NeuN stained by NeuN antibody (GTX132974) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX132974/GTX132974_44986_20230519_ICC_IF_R_23053001_624.webp)
