NeuN antibody detects NeuN protein at nucleus by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NeuN stained by NeuN antibody (GTX132974) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
![NeuN antibody detects NeuN protein at nucleus by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: NeuN protein stained by NeuN antibody (GTX132974) diluted at 1:1000. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX132974/GTX132974_42564_20170503_IFA_R_w_23060523_597.webp "NeuN antibody detects NeuN protein at nucleus by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: NeuN protein stained by NeuN antibody (GTX132974) diluted at 1:1000. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![NeuN antibody detects NeuN protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded 3xTg-AD transgenic mouse brain (left) and healthy mouse brain (right). Red: NeuN stained by NeuN antibody (GTX132974) diluted at 1:250. Green: Beta amyloid (1-42) stained by Beta amyloid (1-42) antibody – Conformation Specific antibody [GT622] (GTX635160) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min This image was provided courtesy of a customer review.](https://www.genetex.com/upload/website/prouct_img/normal/GTX132974/GTX132974_42564_20190510_IHC-P-FL_M_w_23060523_297.webp "NeuN antibody detects NeuN protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded 3xTg-AD transgenic mouse brain (left) and healthy mouse brain (right). Red: NeuN stained by NeuN antibody (GTX132974) diluted at 1:250. Green: Beta amyloid (1-42) stained by Beta amyloid (1-42) antibody – Conformation Specific antibody [GT622] (GTX635160) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min This image was provided courtesy of a customer review.")
![NeuN antibody detects NeuN protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: NeuN protein stained by NeuN antibody (GTX132974) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX132974/GTX132974_42564_20170531_IHC-Fr_M_w_23060523_819.webp "NeuN antibody detects NeuN protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: NeuN protein stained by NeuN antibody (GTX132974) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![NeuN antibody detects NeuN protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: NeuN protein stained by NeuN antibody (GTX132974) diluted at 1:250. Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX132974/GTX132974_42564_42886_IHC-Fr_M_w_23060523_317.webp "NeuN antibody detects NeuN protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: NeuN protein stained by NeuN antibody (GTX132974) diluted at 1:250. Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.")
NeuN antibody detects NeuN protein at nucleus by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NeuN stained by NeuN antibody (GTX132974) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
NeuN antibody
GTX132974
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityFish, Human, Mouse, Rat
TargetRBFOX3
Overview
- SupplierGeneTex
- Product NameNeuN antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.47 mg/ml
- ConjugateUnconjugated
- Gene ID146713
- Target nameRBFOX3
- Target descriptionRNA binding fox-1 homolog 3
- Target synonymsFOX-3, FOX3, HRNBP3, NEUN, RNA binding protein fox-1 homolog 3, RNA binding protein, fox-1 homolog 3, fox-1 homolog C, hexaribonucleotide binding protein 3, neuN antigen, neuronal nuclei antigen
- HostRabbit
- IsotypeIgG
- Protein IDA6NFN3
- Protein NameRNA binding protein fox-1 homolog 3
- ReactivityFish, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Feng T, Du H, Hu F. Loss of TMEM106B exacerbates Tau pathology and neurodegeneration in PS19 mice. bioRxiv. 2023,:pii: 2023.11.11.566707. doi: 10.1101/2023.11.11.566707.Read this paper
- Montillot C, Skutunova E, Ayushma, et al. Characterization of Vps13b-mutant mice reveals neuroanatomical and behavioral phenotypes with females less affected. Neurobiol Dis. 2023,185:106259. doi: 10.1016/j.nbd.2023.106259Read this paper
- Liang MZ, Lu TH, Chen L. Timely expression of PGAM5 and its cleavage control mitochondrial homeostasis during neurite re-growth after traumatic brain injury. Cell Biosci. 2023,13(1):96. doi: 10.1186/s13578-023-01052-0Read this paper
- Hayashi Y, Otsuji J, Oshima E, et al. Microglia cause structural remodeling of noradrenergic axon in the trigeminal spinal subnucleus caudalis after infraorbital nerve injury in rats. Brain Behav Immun Health. 2023,30:100622. doi: 10.1016/j.bbih.2023.100622Read this paper
- Lu Y, Liu M, Guo X, et al. miR-26a-5p alleviates CFA-induced chronic inflammatory hyperalgesia through Wnt5a/CaMKII/NFAT signaling in mice. CNS Neurosci Ther. 2023,29(5):1254-1271. doi: 10.1111/cns.14099Read this paper
- Huang DF, Lee CY, Chou MY, et al. Neuronal splicing regulator RBFOX3 mediates seizures via regulating Vamp1 expression preferentially in NPY-expressing GABAergic neurons. Proc Natl Acad Sci U S A. 2022,119(33):e2203632119. doi: 10.1073/pnas.2203632119Read this paper
- Jhan MK, Chen CL, Shen TJ, et al. Polarization of Type 1 Macrophages Is Associated with the Severity of Viral Encephalitis Caused by Japanese Encephalitis Virus and Dengue Virus. Cells. 2021,10(11). doi: 10.3390/cells10113181Read this paper
- Huang J, Tan YX, Xue LL, et al. Panax notoginseng saponin attenuates the hypoxic-ischaemic injury in neonatal rats by regulating the expression of neurotrophin factors. Eur J Neurosci. 2021,54(6):6304-6321. doi: 10.1111/ejn.15428Read this paper
- Xiong LL, Tan YX, Du RL, et al. Effect of Sutellarin on Neurogenesis in Neonatal Hypoxia-Ischemia Rat Model: Potential Mechanisms of Action. Am J Chin Med. 2021,49(3):677-703. doi: 10.1142/S0192415X21500312Read this paper
- Chuang HC, Chen HC, Chai PJ, et al. Neuropathology changed by 3- and 6-months low-level PM(2.5) inhalation exposure in spontaneously hypertensive rats. Part Fibre Toxicol. 2020,17(1):59. doi: 10.1186/s12989-020-00388-6Read this paper
Datasheet
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