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Western Blot analysis of various samples using FOXP3 Polyclonal Antibody at dilution of 1:800.
Western Blot analysis of various samples using FOXP3 Polyclonal Antibody at dilution of 1:800.
Western Blot analysis of various samples using FOXP3 Polyclonal Antibody at dilution of 1:800.

FOXP3 Polyclonal Antibody

E-AB-70038
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    FOXP3 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Foxp3 using anti-Foxp3 antibody (A00011-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Foxp3 antigen affinity purified polyclonal antibody (Catalog # A00011-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Foxp3 at approximately 50 kDa. The expected band size for Foxp3 is at 50 kDa.
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