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Western Blot Positive WB detected in: Mouse lung tissue, Mouse stomach tissue All lanes: GAD2 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 66 kDa Observed band size: 66 kDa
Western Blot Positive WB detected in: Mouse lung tissue, Mouse stomach tissue All lanes: GAD2 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 66 kDa Observed band size: 66 kDa
Western Blot Positive WB detected in: Mouse lung tissue, Mouse stomach tissue All lanes: GAD2 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 66 kDa Observed band size: 66 kDa

GAD2 Antibody

CSB-PA11159A0RB
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGAD2
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Overview

  • Supplier
    Cusabio
  • Product Name
    GAD2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2572
  • Target name
    GAD2
  • Target description
    glutamate decarboxylase 2
  • Target synonyms
    65 kDa glutamic acid decarboxylase; GAD65; GAD-65; glutamate decarboxylase 2; glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); Glutamate decarboxylase-2 (pancreas)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ05329
  • Protein Name
    Glutamate decarboxylase 2
  • Scientific Description
    Catalyzes the production of GABA.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GAD65 using anti-GAD65 antibody (A03142-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAD65 antigen affinity purified polyclonal antibody (Catalog # A03142-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAD65 at approximately 65 kDa. The expected band size for GAD65 is at 65 kDa.
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