More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/GAD65 Antibody

GAD65 Antibody

LS-C405593
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGAD2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GAD65 Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:25 - 1:100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2572
  • Target name
    GAD2
  • Target description
    glutamate decarboxylase 2
  • Target synonyms
    65 kDa glutamic acid decarboxylase; GAD65; GAD-65; glutamate decarboxylase 2; glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); Glutamate decarboxylase-2 (pancreas)
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Everest Biotech
Goat anti-GAD2 / GAD65EB06730
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetGAD2
  • SizePrice
Product group Antibodies
Biomatik
Gad2 Polyclonal AntibodyCAC08038
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetGAD2
  • SizePrice
SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GAD65) polyclonal Antibody, Unconjugated (bs-0400R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Product group Antibodies
References
Bioss Antibodies
GAD65 Polyclonal AntibodyBS-0400R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetGAD2
  • SizePrice
Product group Antibodies
Assay Genie
GAD2 AntibodyCAB0971
TargetGAD2
  • SizePrice
Product group Antibodies
MedChem Express
GAD65 Antibody (YA758)HY-P80682
TargetGAD2
  • SizePrice
Product group Antibodies
GeneTex
GAD65 antibody [C2C3], C-termGTX100281
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetGAD2
  • SizePrice
Western Blot Positive WB detected in: Mouse lung tissue, Mouse stomach tissue All lanes: GAD2 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 66 kDa Observed band size: 66 kDa
Product group Antibodies
Cusabio
GAD2 AntibodyCSB-PA11159A0RB
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetGAD2
  • SizePrice
Immunohistochemistry analysis in human cerebral cortex and tonsil tissues using AMAb91048 antibody. Corresponding GAD2 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-GAD2 AntibodyAMAB91048
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetGAD2
  • SizePrice
Product group Antibodies
RayBiotech
Anti-GAD2 Antibody144-00971
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetGAD2
  • SizePrice
Figure 1. Western blot analysis of GAD65 using anti-GAD65 antibody (A03142-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAD65 antigen affinity purified polyclonal antibody (Catalog # A03142-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAD65 at approximately 65 kDa. The expected band size for GAD65 is at 65 kDa.
Product group Antibodies
Boster Bio
Anti-GAD65/GAD2 Antibody Picoband(r)A03142-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetGAD2
  • SizePrice