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Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using Galectin3(GTX113486) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using Galectin3(GTX113486) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using Galectin3(GTX113486) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

Galectin 3 antibody

GTX113486
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Rat
TargetLGALS3
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Overview

  • Supplier
    GeneTex
  • Product Name
    Galectin 3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Sandwich ELISA: . *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.85 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3958
  • Target name
    LGALS3
  • Target description
    galectin 3
  • Target synonyms
    CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2, galectin-3, 35 kDa lectin, IgE-binding protein, MAC-2 antigen, advanced glycation end-product receptor 3, carbohydrate-binding protein 35, epididymis secretory sperm binding protein, galactose-specific lectin 3, laminin-binding protein, lectin L-29, lectin, galactoside-binding, soluble, 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17931
  • Protein Name
    Galectin-3
  • Scientific Description
    This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.
  • Reactivity
    Canine, Human, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Liu X, Wang Y, Zhang M, et al. Ticagrelor Reduces Ischemia-Reperfusion Injury Through the NF-κB-Dependent Pathway in Rats. J Cardiovasc Pharmacol. 2019,74(1):13-19. doi: 10.1097/FJC.0000000000000675
    Read this paper
  • Hönig E, Ringer K, Dewes J, et al. Galectin-3 modulates the polarized surface delivery of β1-integrin in epithelial cells. J Cell Sci. 2018,131(11):pii: jcs213199. doi: 10.1242/jcs.213199.
    Read this paper
  • Liu X, Gu Y, Liu Y, et al. Ticagrelor attenuates myocardial ischaemia-reperfusion injury possibly through downregulating galectin-3 expression in the infarct area of rats. Br J Clin Pharmacol. 2018,84(6):1180-1186. doi: 10.1111/bcp.13536
    Read this paper

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Figure 1. Western blot analysis of Galectin-3/LGALS3 using anti-Galectin-3/LGALS3 antibody (A00621-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin-3/LGALS3 antigen affinity purified polyclonal antibody (Catalog # A00621-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin-3/LGALS3 at approximately 29 kDa. The expected band size for Galectin-3/LGALS3 is at 26 kDa.
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