IHC-P analysis of human breast carcinoma tissue using GTX15794 gamma Tubulin antibody [4D11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
![IHC-P analysis of human colon tissue using GTX15794 gamma Tubulin antibody [4D11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX15794/GTX15794_1045_IHC-P_w_23060620_736.webp "IHC-P analysis of human colon tissue using GTX15794 gamma Tubulin antibody [4D11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![WB analysis of gamma tubulin knockdown HeLa cells (lane 3), scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1) using GTX15794 gamma Tubulin antibody [4D11]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX15794/GTX15794_1539_WB_w_23060620_798.webp "WB analysis of gamma tubulin knockdown HeLa cells (lane 3), scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1) using GTX15794 gamma Tubulin antibody [4D11]. Dilution : 1:1000")
![WB analysis of 50 ug of various whole cell lysates using GTX15794 gamma Tubulin antibody [4D11]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX15794/GTX15794_1540_WB_w_23060620_499.webp "WB analysis of 50 ug of various whole cell lysates using GTX15794 gamma Tubulin antibody [4D11]. Dilution : 1:1000")
![WB analysis of membrane enriched extracts (30 ug lysate) of A-549 (Lane 1), MCF-7 (Lane 2), HeLa (Lane 3), HepG2 (Lane 4), Caco2 (Lane 5), A-431 (Lane 6), SH-SY5Y (Lane 7) and U-2 OS (Lane 8) using GTX15794 gamma Tubulin antibody [4D11]. Dilution : 1 microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX15794/GTX15794_1541_WB_w_23060620_376.webp "WB analysis of membrane enriched extracts (30 ug lysate) of A-549 (Lane 1), MCF-7 (Lane 2), HeLa (Lane 3), HepG2 (Lane 4), Caco2 (Lane 5), A-431 (Lane 6), SH-SY5Y (Lane 7) and U-2 OS (Lane 8) using GTX15794 gamma Tubulin antibody [4D11]. Dilution : 1 microg/ml")
![ICC/IF analysis of HeLa (A) and U2-OS cells (B) using GTX15794 gamma Tubulin antibody [4D11]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX15794/GTX15794_319_ICC-IF_w_23060620_584.webp "ICC/IF analysis of HeLa (A) and U2-OS cells (B) using GTX15794 gamma Tubulin antibody [4D11]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature")
IHC-P analysis of human breast carcinoma tissue using GTX15794 gamma Tubulin antibody [4D11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
gamma Tubulin antibody [4D11]
GTX15794
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Primate, Rat
TargetTUBG1
Overview
- SupplierGeneTex
- Product Namegamma Tubulin antibody [4D11] - KO/KD Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. ICC/IF: 1:50. IHC-P: 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4D11
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7283
- Target nameTUBG1
- Target descriptiontubulin gamma 1
- Target synonymsCDCBM4; gamma-tubulin complex component 1; GCP-1; TUBG; TUBGCP1; tubulin gamma-1 chain; tubulin, gamma polypeptide
- HostMouse
- IsotypeIgG2b
- Protein IDP23258
- Protein NameTubulin gamma-1 chain
- Scientific DescriptionThis gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq, Jan 2009]
- ReactivityHuman, Mouse, Primate, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203