ICC/IF analysis of NIH3T3 cells using GTX11317 gamma Tubulin antibody at 1:500 (green).Actin staining with phalloidin rhodamine conjugate(red). Nuclear staining with DAPI(blue).
ICC/IF analysis of NIH3T3 cells using GTX11317 gamma Tubulin antibody at 1:500 (green).Actin staining with phalloidin rhodamine conjugate(red). Nuclear staining with DAPI(blue).
gamma Tubulin antibody
GTX11317
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityChicken, Hamster, Human, Mouse, Rat
TargetTUBG1
Overview
- SupplierGeneTex
- Product Namegamma Tubulin antibody
- Delivery Days Customer9
- Antibody SpecificityAnti-gamma-Tubulin recognizes an epitope located in the N-terminal region of gamma-tubulin (amino acids 38-53). Staining of gamma-tubulin (48 kDa) by immunoblotting is specifically inhibited with gamma-tubulin peptide (human, amino acids 38-53 with C-term
- Application Supplier NoteWB: 1:1,000. ICC/IF: 1:5,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID7283
- Target nameTUBG1
- Target descriptiontubulin gamma 1
- Target synonymsCDCBM4; gamma-tubulin complex component 1; GCP-1; TUBG; TUBGCP1; tubulin gamma-1 chain; tubulin, gamma polypeptide
- HostRabbit
- IsotypeIgG
- Protein IDP23258
- Protein NameTubulin gamma-1 chain
- Scientific DescriptionThe protein that binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome is called gamma tubulin. Gamma Tubulin functions as the microtubule nucleator at the microtubule organizing centre (MTOC). By binding to the beta tubulin half of the tubulin molecule, it establishes the polarity of a microtubule, leaving the alpha tubulin half exposed at the positive end. Gamma Tubulin (approx. 48 kD) is a ubiquitous and highly conserved protein within the MTOCs in eukaryotic kingdom. It is related to alpha and beta tubulin and is, thus, a member of the tubulin superfamily of proteins. However, its abundance is less than 1% of the level of either alpha or beta tubulin. Moreover, unlike alpha and beta tubulin, it is not a component of microtubules. Rather, it is located at the MTOC. gamma Tubulin shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton. Antibodies reacting specifically with gamma tubulin serve as an essential tool in the detection of the presence and role of this molecule in various cellular settings.
- ReactivityChicken, Hamster, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203