ICC/IF analysis of HeLa cells using GTX11316 gamma Tubulin antibody [GTU-88] at 1:5,000. Cells were fixed and permeabilized with methanol followed by acetone.
![WB analysis of chicken fibroblasts using GTX11316 gamma Tubulin antibody [GTU-88]. Lane A : Antibody dilution 1:20,000 Lane B : Negative control (secondary antibody only)](https://www.genetex.com/upload/website/prouct_img/normal/GTX11316/GTX11316_20170605_WB_1_w_23060500_319.webp "WB analysis of chicken fibroblasts using GTX11316 gamma Tubulin antibody [GTU-88]. Lane A : Antibody dilution 1:20,000 Lane B : Negative control (secondary antibody only)")
![WB analysis of (1) HeLa (2) U87 (3) COS7 (4) P19 (5) rat2 (6) CHO (7) MDBK (8) MDCK cell lysate using GTX11316 gamma Tubulin antibody [GTU-88] at 1:10,000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX11316/GTX11316_20170605_WB_w_23060501_973.webp "WB analysis of (1) HeLa (2) U87 (3) COS7 (4) P19 (5) rat2 (6) CHO (7) MDBK (8) MDCK cell lysate using GTX11316 gamma Tubulin antibody [GTU-88] at 1:10,000.")
ICC/IF analysis of HeLa cells using GTX11316 gamma Tubulin antibody [GTU-88] at 1:5,000. Cells were fixed and permeabilized with methanol followed by acetone.
gamma Tubulin antibody [GTU-88]
GTX11316
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityCanine, Chicken, Drosophila, Hamster, Human, Monkey, Mouse, Rat, Xenopus
TargetTUBG1
Overview
- SupplierGeneTex
- Product Namegamma Tubulin antibody [GTU-88]
- Delivery Days Customer9
- Antibody SpecificityMonoclonal Anti-gamma-Tubulin recognizes an epitope located in the N-terminal amino acids 38-53 of gamma-tubulin (48 kDa). Cross reactivity has been observed with human, bovine, dog, hamster, rat, mouse, chicken, and Xenopus gamma-tubulin.
- Application Supplier NoteWB: 1:10,000. ICC/IF: 1:5,000-1:10,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGTU-88
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID7283
- Target nameTUBG1
- Target descriptiontubulin gamma 1
- Target synonymsCDCBM4; gamma-tubulin complex component 1; GCP-1; TUBG; TUBGCP1; tubulin gamma-1 chain; tubulin, gamma polypeptide
- HostMouse
- IsotypeIgG1
- Protein IDP23258
- Protein NameTubulin gamma-1 chain
- Scientific DescriptionThe protein that binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome is called gamma tubulin. Gamma Tubulin functions as the microtubule nucleator at the microtubule organizing centre (MTOC). By binding to the beta tubulin half of the tubulin molecule, it establishes the polarity of a microtubule, leaving the alpha tubulin half exposed at the positive end. Gamma Tubulin (approx. 48 kD) is a ubiquitous and highly conserved protein within the MTOCs in eukaryotic kingdom. It is related to alpha and beta tubulin and is, thus, a member of the tubulin superfamily of proteins. However, its abundance is less than 1% of the level of either alpha or beta tubulin. Moreover, unlike alpha and beta tubulin, it is not a component of microtubules. Rather, it is located at the MTOC. gamma Tubulin shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton. Antibodies reacting specifically with gamma tubulin serve as an essential tool in the detection of the presence and role of this molecule in various cellular settings.
- ReactivityCanine, Chicken, Drosophila, Hamster, Human, Monkey, Mouse, Rat, Xenopus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Src-mediated tyrosine phosphorylation of PRC1 and kinastrin/SKAP on the mitotic spindle. Morii M et al., 2021 Jan 28, Sci RepRead more
- Functional Alterations in Ciliogenesis-Associated Kinase 1 (CILK1) that Result from Mutations Linked to Juvenile Myoclonic Epilepsy. Wang EJ et al., 2020 Mar 12, CellsRead more
- Diverse mitotic functions of the cytoskeletal cross-linking protein Shortstop suggest a role in Dynein/Dynactin activity. Dewey EB et al., 2017 Sep 15, Mol Biol CellRead more
- Ran-dependent TPX2 activation promotes acentrosomal microtubule nucleation in neurons. Chen WS et al., 2017 Feb 13, Sci RepRead more
- Involvement of clusterin and the aggresome in abnormal protein deposits in myofibrillar myopathies and inclusion body myositis. Ferrer I et al., 2005 Apr, Brain PatholRead more