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GAPDH antibody

10-1500
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    GAPDH antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GAPDH using anti-GAPDH antibody (A00227-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human CCRF-CEM whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (Catalog # A00227-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.
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293T cell lysates probed with different dilutions of GAPDH Polyconal Antibody (bs-10900R). Lane 1: 1:2000; Lane 2: 1:10,000; Lane 3: 1:40,000, Lane 4: 1:80,000 antibody incubated overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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References
Bioss
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