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IHC-P analysis of human cerebellum tissue using GTX81794 GARP antibody, Internal.
IHC-P analysis of human cerebellum tissue using GTX81794 GARP antibody, Internal.
IHC-P analysis of human cerebellum tissue using GTX81794 GARP antibody, Internal.

GARP antibody, Internal

GTX81794
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetLRRC32
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Overview

  • Supplier
    GeneTex
  • Product Name
    GARP antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. IHC-P: 1:10-1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2615
  • Target name
    LRRC32
  • Target description
    leucine rich repeat containing 32
  • Target synonyms
    CPPRDD; D11S833E; GARP; garpin; glycoprotein A repetitions predominant; leucine-rich repeat-containing protein 32; transforming growth factor beta activator LRRC32
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14392
  • Protein Name
    Leucine-rich repeat-containing protein 32
  • Scientific Description
    This gene encodes a type I membrane protein which contains 20 leucine-rich repeats. Alterations in the chromosomal region 11q13-11q14 are involved in several pathologies. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Selective activation of TGFbeta signaling by P. gingivalis-mediated upregulation of GARP aggravates esophageal squamous cell carcinoma.
    Read more

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Figure 1. Western blot analysis of LRRC32 using anti-LRRC32 antibody (A08199-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SK-OV-3 whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC32 antigen affinity purified polyclonal antibody (Catalog # A08199-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRC32 at approximately 72KD. The expected band size for LRRC32 is at 72KD.
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