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WB analysis of various samples using GTX66705 GCLC antibody. Lane1 : HepG2 whole cell lysate(40ug) Lane2 : NIH-3T3 whole cell lysate(40ug) Lane3 : PC12 whole cell lysate(40ug) Lane4 : A549 whole cell lysate(40ug) Dilution : 1:500
WB analysis of various samples using GTX66705 GCLC antibody. Lane1 : HepG2 whole cell lysate(40ug) Lane2 : NIH-3T3 whole cell lysate(40ug) Lane3 : PC12 whole cell lysate(40ug) Lane4 : A549 whole cell lysate(40ug) Dilution : 1:500
WB analysis of various samples using GTX66705 GCLC antibody. Lane1 : HepG2 whole cell lysate(40ug) Lane2 : NIH-3T3 whole cell lysate(40ug) Lane3 : PC12 whole cell lysate(40ug) Lane4 : A549 whole cell lysate(40ug) Dilution : 1:500

GCLC antibody

GTX66705
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetGCLC
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Overview

  • Supplier
    GeneTex
  • Product Name
    GCLC antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2729
  • Target name
    GCLC
  • Target description
    glutamate-cysteine ligase catalytic subunit
  • Target synonyms
    gamma-ECS; gamma-glutamylcysteine synthetase; GCL; GCS; GCS heavy chain; GLCL; GLCLC; glutamate--cysteine ligase catalytic subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48506
  • Protein Name
    Glutamate--cysteine ligase catalytic subunit
  • Scientific Description
    Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GCLC using anti-GCLC antibody (A01722). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCLC antigen affinity purified polyclonal antibody (Catalog # A01722) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GCLC at approximately 73 kDa. The expected band size for GCLC is at 73 kDa.
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