IHC-P analysis of human bronchial epithelial tissue using GTX16315 GCLC antibody at 1:600. Left to right : DAPI, GCLC, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer.
IHC-P analysis of human bronchial epithelial tissue using GTX16315 GCLC antibody at 1:600. Left to right : DAPI, GCLC, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer.
GCLC antibody, N-term
GTX16315
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetGCLC
Overview
- SupplierGeneTex
- Product NameGCLC antibody, N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 0.2-2.5 ug/ml. IHC-P: 2-10 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.5-1 mg/ml
- ConjugateUnconjugated
- Gene ID2729
- Target nameGCLC
- Target descriptionglutamate-cysteine ligase catalytic subunit
- Target synonymsgamma-ECS; gamma-glutamylcysteine synthetase; GCL; GCS; GCS heavy chain; GLCL; GLCLC; glutamate--cysteine ligase catalytic subunit
- HostRabbit
- IsotypeIgG
- Protein IDP48506
- Protein NameGlutamate--cysteine ligase catalytic subunit
- Scientific DescriptionGlutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
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- Zerumbone Exhibits Antiphotoaging and Dermatoprotective Properties in Ultraviolet A-Irradiated Human Skin Fibroblast Cells via the Activation of Nrf2/ARE Defensive Pathway. Hseu YC et al., 2019, Oxid Med Cell LongevRead more
- The Protective Effect of Hispidin against Hydrogen Peroxide-Induced Oxidative Stress in ARPE-19 Cells via Nrf2 Signaling Pathway. Huang SY et al., 2019 Aug 19, BiomoleculesRead more
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- The in vitro and in vivo depigmenting activity of Coenzyme Q10 through the down-regulation of alpha-MSH signaling pathways and induction of Nrf2/ARE-mediated antioxidant genes in UVA-irradiated skin keratinocytes. Hseu YC et al., 2019 Jun, Biochem PharmacolRead more
- Trans-cinnamic acid attenuates UVA-induced photoaging through inhibition of AP-1 activation and induction of Nrf2-mediated antioxidant genes in human skin fibroblasts. Hseu YC et al., 2018 May, J Dermatol SciRead more
- Zerumbone protects human skin keratinocytes against UVA-irradiated damages through Nrf2 induction. Yang HL et al., 2018 Feb, Biochem PharmacolRead more
- Caffeic acid and resveratrol ameliorate cellular damage in cell and Drosophila models of spinocerebellar ataxia type 3 through upregulation of Nrf2 pathway. Wu YL et al., 2018 Feb 1, Free Radic Biol MedRead more