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ProductsBack/Gelsolin antibody [HL1931]
Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [HL1931] (GTX637768) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [HL1931] (GTX637768) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [HL1931] (GTX637768) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Gelsolin antibody [HL1931]

GTX637768
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetGSN
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Overview

  • Supplier
    GeneTex
  • Product Name
    Gelsolin antibody [HL1931]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL1931
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2934
  • Target name
    GSN
  • Target description
    gelsolin
  • Target synonyms
    ADF, AGEL, AMYLD4, gelsolin, actin-depolymerizing factor, brevin, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06396
  • Protein Name
    Gelsolin
  • Scientific Description
    The protein encoded by this gene binds to the plus ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Gelsolin using anti-Gelsolin antibody (PB9209). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: monkey kidney tissue lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gelsolin antigen affinity purified polyclonal antibody (Catalog # PB9209) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gelsolin at approximately 86 kDa. The expected band size for Gelsolin is at 86 kDa.
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