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ProductsBack/GHR (MAB 5): m-IgG Fc BP-HRP Bundle

GHR (MAB 5): m-IgG Fc BP-HRP Bundle

SC-539255
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetGHR
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    GHR (MAB 5): m-IgG Fc BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2690
  • Target name
    GHR
  • Target description
    growth hormone receptor
  • Target synonyms
    GHBP, GHIP, growth hormone receptor, GH receptor, growth hormone binding protein, mutant growth hormone receptor, serum binding protein, somatotropin receptor
  • Host
    Mouse
  • Isotype
    IgG2b
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GHR using anti-GHR antibody (A00698). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GHR antigen affinity purified polyclonal antibody (Catalog # A00698) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GHR at approximately 80KD. The expected band size for GHR is at 72KD.
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