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ProductsBack/GLI / GLI1 Antibody (Preservative Free)

GLI / GLI1 Antibody (Preservative Free)

LS-C815605
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGLI1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GLI / GLI1 Antibody (Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ICC (1:200 - 1:000), IF (1:200 - 1:1000), IHC-P (1:100 - 1:500) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2735
  • Target name
    GLI1
  • Target description
    GLI family zinc finger 1
  • Target synonyms
    GLI, PAPA8, PPD1, zinc finger protein GLI1, GLI-Kruppel family member GLI1, glioma-associated oncogene 1, glioma-associated oncogene homolog 1 (zinc finger protein), oncogene GLI
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GLI1 using anti-GLI1 antibody (A00527-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLI1 antigen affinity purified polyclonal antibody (Catalog # A00527-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLI1 at approximately 150 kDa. The expected band size for GLI1 is at 118 kDa.
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Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GLI1 Polyclonal Antibody, Unconjugated (bs-1206R) at 1:400 overnight at 4°C, DAB staining.
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