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GLI1 Antibody

CAB14675
Assay Genie
TargetGLI1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    GLI1 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID2735
  • Target name
    GLI1
  • Target description
    GLI family zinc finger 1
  • Target synonyms
    GLI; GLI-Kruppel family member GLI1; glioma-associated oncogene 1; glioma-associated oncogene homolog 1 (zinc finger protein); oncogene GLI; PAPA8; PPD1; zinc finger protein GLI1
  • Host
    Rabbit
  • Protein IDP08151
  • Protein Name
    Zinc finger protein GLI1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GLI1 using anti-GLI1 antibody (A00527-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLI1 antigen affinity purified polyclonal antibody (Catalog # A00527-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLI1 at approximately 150 kDa. The expected band size for GLI1 is at 118 kDa.
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Immunofluorescence staining of RH-30 cells using the Anti-GLI1 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
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