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Glucocorticoid Receptor alpha Antibody (YA754, Mouse)

HY-P80143
MedChem Express
TargetNR3C1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Glucocorticoid Receptor alpha Antibody (YA754, Mouse)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2908
  • Target name
    NR3C1
  • Target description
    nuclear receptor subfamily 3 group C member 1
  • Target synonyms
    GCCR, GCR, GCRST, GR, GRL, glucocorticoid receptor, nuclear receptor subfamily 3 group C member 1 variant hGR-B(54), nuclear receptor subfamily 3 group C member 1 variant hGR-B(77), nuclear receptor subfamily 3 group C member 1 variant hGR-B(93), nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Glucocorticoid Receptor alpha Antibody (YA754) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Glucocorticoid Receptor alpha.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NR3C1 using anti-NR3C1 antibody (PB9232). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR3C1 antigen affinity purified polyclonal antibody (Catalog # PB9232) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR3C1 at approximately 95 kDa. The expected band size for NR3C1 is at 86 kDa.
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