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Formalin-fixed and paraffin embedded: human lung epithelial cells labeled with Anti-Glucocorticoid receptor(GR) Polyclonal Antibody, Unconjugated (bs-0252R) at 1:200,, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) at 1:200 for 40 minutes at 37°C
Formalin-fixed and paraffin embedded: human lung epithelial cells labeled with Anti-Glucocorticoid receptor(GR) Polyclonal Antibody, Unconjugated (bs-0252R) at 1:200,, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) at 1:200 for 40 minutes at 37°C
Formalin-fixed and paraffin embedded: human lung epithelial cells labeled with Anti-Glucocorticoid receptor(GR) Polyclonal Antibody, Unconjugated (bs-0252R) at 1:200,, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) at 1:200 for 40 minutes at 37°C

Glucocorticoid receptor Antibody

BS-0252R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNR3C1
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Overview

  • Supplier
    Bioss
  • Product Name
    Glucocorticoid receptor Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2908
  • Target name
    NR3C1
  • Target description
    nuclear receptor subfamily 3 group C member 1
  • Target synonyms
    GCCR, GCR, GCRST, GR, GRL, glucocorticoid receptor, nuclear receptor subfamily 3 group C member 1 variant hGR-B(54), nuclear receptor subfamily 3 group C member 1 variant hGR-B(77), nuclear receptor subfamily 3 group C member 1 variant hGR-B(93), nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04150
  • Protein Name
    Glucocorticoid receptor
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Zoratto F, Sbriccoli M, Martinelli A, et al. Intranasal oxytocin administration promotes emotional contagion and reduces aggression in a mouse model of callousness. Neuropharmacology. 2018,143:250-267. doi: 10.1016/j.neuropharm.2018.09.010
    Read this paper
  • Guo J, Ma K, Xia HM, et al. Androgen receptor reverts dexamethasone‑induced inhibition of prostate cancer cell proliferation and migration. Mol Med Rep. 2018,17(4):5887-5893. doi: 10.3892/mmr.2018.8566
    Read this paper
  • Pang X, Zhang P, Zhu S, et al. Expression of glucocorticoid receptor-α in the epidermis of patients with psoriasis vulgaris. Exp Ther Med. 2015,10(2):419-422.
    Read this paper

Datasheet

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Figure 1. Western blot analysis of NR3C1 using anti-NR3C1 antibody (PB9232). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR3C1 antigen affinity purified polyclonal antibody (Catalog # PB9232) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR3C1 at approximately 95 kDa. The expected band size for NR3C1 is at 86 kDa.
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