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Glutathione S Transferase mu 1 Antibody (YA2308, Rabbit)

HY-P82563
MedChem Express
TargetGSTM1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Glutathione S Transferase mu 1 Antibody (YA2308, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2944
  • Target name
    GSTM1
  • Target description
    glutathione S-transferase mu 1
  • Target synonyms
    GST1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GTM1, H-B, MU, MU-1, glutathione S-transferase Mu 1, GST HB subunit 4, GST class-mu 1, HB subunit 4, S-(hydroxyalkyl)glutathione lyase, glutathione S-alkyltransferase, glutathione S-aralkyltransferase, glutathione S-aryltransferase, glutathione S-transferase M1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Glutathione S Transferase mu 1 Antibody (YA2308) is a non-conjugated IgG antibody, targeting GSTM1, with a predicted molecular weight of 26 kDa (observed band size: 26 kDa). Glutathione S Transferase mu 1 Antibody (YA2308) can be used for WB experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GSTM1 using anti-GSTM1 antibody (A00569-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse stomach tissue lysates, Lane 4: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTM1 antigen affinity purified polyclonal antibody (Catalog # A00569-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSTM1 at approximately 26KD. The expected band size for GSTM1 is at 26KD.
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