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Goat anti-ATP6IP2 / Renin receptor

EB06118
Everest Biotech
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
TargetATP6AP2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ATP6IP2 / Renin receptor Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Flow Cytometry: Flow cytometric analysis of HeLa cells.. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID10159
  • Target name
    ATP6AP2
  • Target description
    ATPase H+ transporting accessory protein 2
  • Target synonyms
    APT6M8-9; ATP6IP2; ATP6M8-9; ATPase H(+)-transporting lysosomal-interacting protein 2; ATPase, H+ transporting, lysosomal (vacuolar proton pump) membrane sector associated protein M8-9; ATPase, H+ transporting, lysosomal accessory protein 2; ATPase, H+ transporting, lysosomal interacting protein 2; CDG2R; ELDF10; embryonic liver differentiation factor 10; ER-localized type I transmembrane adapter; ER-localized type I transmembrane adaptor; HT028; M8-9; MRXE; MRXSH; MSTP009; N14F; prorenin receptor; PRR; renin receptor; renin/prorenin receptor; RENR; vacuolar ATP synthase membrane sector-associated protein M8-9; vacuolar proton ATP synthase membrane sector associated protein M8-9; V-ATPase M8.9 subunit; XMRE; XPDS
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_005756.2. GeneIDs all Nonhuman: 70495 (mouse);. Purification: Antigen affinity purified. Names and symbols: ATP6AP2; ATP6IP2; ATPase, H+ transporting, lysosomal interacting protein 2; M8-9; APT6M8-9; ATP6M8-9; renin receptor; V-ATPase M8.9 subunit; ATPase membrane sector associated protein M8-9; vacuolar ATP synthase membrane sector associated protein M8-9; ATP
  • Reactivity Supplier
    Human, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Expression of renin-angiotensin system (RAS) components in endometrial cancer. Delforce SJ et al., 2017 Jan, Endocr Connect
    Read more
  • Molecular evidence of a (pro)renin/ (pro)renin receptor system in human intrauterine tissues in pregnancy and its association with PGHS-2. Pringle KG et al., 2011 Sep, J Renin Angiotensin Aldosterone Syst
    Read more
  • Effect of mineralocorticoid receptor blockade on the renal renin-angiotensin system in Dahl salt-sensitive hypertensive rats. Zhu A et al., 2009 Apr, J Hypertens
    Read more

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Figure 1. Western blot analysis of Renin receptor/ATP6AP2 using anti-Renin receptor/ATP6AP2 antibody (A02665-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human HUVEC whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Renin receptor/ATP6AP2 antigen affinity purified polyclonal antibody (Catalog # A02665-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Renin receptor/ATP6AP2 at approximately 49 kDa. The expected band size for Renin receptor/ATP6AP2 is at 39 kDa.
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