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Renin Receptor antibody [N3C3]

GTX114169
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetATP6AP2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Renin Receptor antibody [N3C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.38 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10159
  • Target name
    ATP6AP2
  • Target description
    ATPase H+ transporting accessory protein 2
  • Target synonyms
    APT6M8-9; ATP6IP2; ATP6M8-9; ATPase H(+)-transporting lysosomal-interacting protein 2; ATPase, H+ transporting, lysosomal (vacuolar proton pump) membrane sector associated protein M8-9; ATPase, H+ transporting, lysosomal accessory protein 2; ATPase, H+ transporting, lysosomal interacting protein 2; CDG2R; ELDF10; embryonic liver differentiation factor 10; ER-localized type I transmembrane adapter; ER-localized type I transmembrane adaptor; HT028; M8-9; MRXE; MRXSH; MSTP009; N14F; prorenin receptor; PRR; renin receptor; renin/prorenin receptor; RENR; vacuolar ATP synthase membrane sector-associated protein M8-9; vacuolar proton ATP synthase membrane sector associated protein M8-9; V-ATPase M8.9 subunit; XMRE; XPDS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75787
  • Protein Name
    Renin receptor
  • Scientific Description
    This gene encodes a protein that is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis. There are three classes of ATPases- F, P, and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. The encoded protein has been found associated with the transmembrane sector of the V-type ATPases. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Cleavage of the V-ATPase associated prorenin receptor is mediated by PACE4 and is essential for growth of prostate cancer cells.
    Read more
  • Elevated V-ATPase Activity Following PTEN Loss Is Required for Enhanced Oncogenic Signaling in Breast Cancer. Mohammad AH et al., 2020 Oct, Mol Cancer Res
    Read more
  • V-ATPase-associated prorenin receptor is upregulated in prostate cancer after PTEN loss. Mohammad AH et al., 2019 Aug 13, Oncotarget
    Read more
  • Elemene inhibits osteosarcoma growth by suppressing the renin-angiotensin system signaling pathway. Ding L et al., 2018 Jan, Mol Med Rep
    Read more
  • Prorenin receptor acts as a potential molecular target for pancreatic ductal adenocarcinoma diagnosis. Arundhathi A et al., 2016 Aug 23, Oncotarget
    Read more
  • Site-1 protease is required for the generation of soluble (pro)renin receptor. Nakagawa T et al., 2017 Apr 1, J Biochem
    Read more
  • Participation of the extracellular domain in (pro)renin receptor dimerization. Suzuki-Nakagawa C et al., 2014 Feb 21, Biochem Biophys Res Commun
    Read more

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Figure 1. Western blot analysis of Renin receptor/ATP6AP2 using anti-Renin receptor/ATP6AP2 antibody (A02665-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human HUVEC whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Renin receptor/ATP6AP2 antigen affinity purified polyclonal antibody (Catalog # A02665-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Renin receptor/ATP6AP2 at approximately 49 kDa. The expected band size for Renin receptor/ATP6AP2 is at 39 kDa.
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