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Goat anti-ATRX

EB07202
Everest Biotech
ApplicationsELISA
Product group Antibodies
TargetATRX
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ATRX Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA
  • Applications Supplier
    Pep-ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID546
  • Target name
    ATRX
  • Target description
    ATRX chromatin remodeler
  • Target synonyms
    JMS, MRX52, RAD54, RAD54L, XH2, XNP, ZNF-HX, transcriptional regulator ATRX, ATP-dependent helicase ATRX, X-linked helicase II, X-linked nuclear protein
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000480.2; NP_612114.1; NP_612115.1. Purification: Antigen affinity purified. Names and symbols: ATRX; alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevisiae); RP5-875J14.1; ATR2; MGC2094; MRXS3; RAD54; RAD54L; SHS; XH2; XNP; ZNF-HX ; DNA dependent ATPase and helicase; OTTHUMP00000062079; RAD54 (Saccharomyces cerevisiae)
  • Reactivity Supplier
    Human, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of ATRX using anti-ATRX antibody (A00203-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A54 whole cell lysates, Lane 2: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATRX antigen affinity purified polyclonal antibody (Catalog # A00203-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATRX at approximately 282KD. The expected band size for ATRX is at 282KD.
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