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ProductsBack/Goat anti-HPK1 / MAP4K1

Goat anti-HPK1 / MAP4K1

EB05454
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMAP4K1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-HPK1 / MAP4K1 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID11184
  • Target name
    MAP4K1
  • Target description
    mitogen-activated protein kinase kinase kinase kinase 1
  • Target synonyms
    HPK1, mitogen-activated protein kinase kinase kinase kinase 1, MAPK/ERK kinase kinase kinase 1, MEK kinase kinase 1, MEKKK 1, hematopoietic progenitor kinase 1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001036065.1; NP_009112.1. Purification: Antigen affinity purified. Names and symbols: MAP4K1; mitogen-activated protein kinase kinase kinase kinase 1; HPK1; hematopoietic progenitor kinase 1
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAP4K1/HPK1 using anti-MAP4K1/HPK1 antibody (A07909-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K1/HPK1 antigen affinity purified polyclonal antibody (Catalog # A07909-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K1/HPK1 at approximately 91 kDa. The expected band size for MAP4K1/HPK1 is at 91 kDa.
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