Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-MAP4K1(Ser171)) Polyclonal Antibody, Unconjugated (bs5494R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
MAP4K1(Ser171) Polyclonal Antibody
BS-5494R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Equine, Human, Mouse, Porcine, Rat
TargetMAP4K1
Overview
- SupplierBioss Antibodies
- Product NameMAP4K1(Ser171) Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID11184
- Target nameMAP4K1
- Target descriptionmitogen-activated protein kinase kinase kinase kinase 1
- Target synonymshematopoietic progenitor kinase 1; HPK1; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; mitogen-activated protein kinase kinase kinase kinase 1
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Protein IDQ92918
- Protein NameMitogen-activated protein kinase kinase kinase kinase 1
- ReactivityCanine, Equine, Human, Mouse, Porcine, Rat
- Storage Instruction-20°C
- UNSPSC12352203