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Western Blot analysis of HepG2 cells using HPK1 Polyclonal Antibody
Western Blot analysis of HepG2 cells using HPK1 Polyclonal Antibody
Western Blot analysis of HepG2 cells using HPK1 Polyclonal Antibody

MAP4K1 Antibody

CSB-PA002967
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMAP4K1
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Overview

  • Supplier
    Cusabio
  • Product Name
    MAP4K1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11184
  • Target name
    MAP4K1
  • Target description
    mitogen-activated protein kinase kinase kinase kinase 1
  • Target synonyms
    hematopoietic progenitor kinase 1; HPK1; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; mitogen-activated protein kinase kinase kinase kinase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92918
  • Protein Name
    Mitogen-activated protein kinase kinase kinase kinase 1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAP4K1/HPK1 using anti-MAP4K1/HPK1 antibody (A07909-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K1/HPK1 antigen affinity purified polyclonal antibody (Catalog # A07909-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K1/HPK1 at approximately 91 kDa. The expected band size for MAP4K1/HPK1 is at 91 kDa.
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