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MAP4K1 Antibody (YA4410, Mouse)

HY-P84713
MedChem Express
TargetMAP4K1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    MAP4K1 Antibody (YA4410, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID11184
  • Target name
    MAP4K1
  • Target description
    mitogen-activated protein kinase kinase kinase kinase 1
  • Target synonyms
    HPK1, mitogen-activated protein kinase kinase kinase kinase 1, MAPK/ERK kinase kinase kinase 1, MEK kinase kinase 1, MEKKK 1, hematopoietic progenitor kinase 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    MAP4K1 Antibody (YA4410) is a mouse-derived and non-conjugated IgG1 antibody, targeting to MAP4K1. It can be applicated for WB, IHC-P, FC, ELISA assays, in the background of human, mouse, monkey, rat.
  • UNSPSC
    41116161
  • Species
    Mouse

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Figure 1. Western blot analysis of MAP4K1/HPK1 using anti-MAP4K1/HPK1 antibody (A07909-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K1/HPK1 antigen affinity purified polyclonal antibody (Catalog # A07909-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K1/HPK1 at approximately 91 kDa. The expected band size for MAP4K1/HPK1 is at 91 kDa.
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