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Goat anti-Htr1a (mouse) Antibody

EB11006
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHTR1A
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Htr1a (mouse) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID3350
  • Target name
    HTR1A
  • Target description
    5-hydroxytryptamine receptor 1A
  • Target synonyms
    5-HT-1A, 5-HT1A, 5HT1a, ADRB2RL1, ADRBRL1, G-21, PFMCD, 5-hydroxytryptamine receptor 1A, 5-HT1a receptor, 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled, guanine nucleotide-binding regulatory protein-coupled receptor
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_032334.2. GeneIDs all Nonhuman: 15550 (mouse); 24473 (rat);. Purification: Antigen affinity purified. Names and symbols: Htr1a; 5-hydroxytryptamine (serotonin) receptor 1A; Gpcr18; 5-HT-1A; 5-HT1A receptor; 5-hydroxytryptamine receptor 1A; G-protein coupled receptor 18; serotonin receptor 1A
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Cordeaux Y, Pasupathy D, Bacon J, et al. Characterization of serotonin receptors in pregnant human myometrium. J Pharmacol Exp Ther. 2009,328(3):682-91. doi: 10.1124/jpet.108.143040
    Read this paper

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Figure 1. Western blot analysis of HTR1A using anti-HTR1A antibody (PA1647). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTR1A antigen affinity purified polyclonal antibody (Catalog # PA1647) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HTR1A at approximately 46 kDa. The expected band size for HTR1A is at 46 kDa.
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